Studies on alkaline protease inhibitor (API-2) produced by Streptomyces griseoincarnatus strain No. KTo-250. IV. Purification of API-2 degrading protease from Streptomyces griseoincarnatus strain No. KTo-250, an API-2 producer.
نویسندگان
چکیده
منابع مشابه
Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
This research has focused on isolation and characterization of a strain of Bacillus <span style="font-variant: normal; font-style: normal; font-family: ArialMT...
متن کاملSecretion of an Alkaline Protease from a Salt- Tolerant and Alkaliphilic, Streptomyces Clavuligerus Strain Mit-1
An alkaliphilic and salttolerant actinomycete, Streptomyces clavuligerus strain Mit-1, was isolated from Mithapur, the western coast of India. The organism was Gram-positive, having filamentous, long thread like structure. The sporulation started after two days of growth and the optimum level of alkaline protease (130 U/ ml) was produced during the early stationary phase. The strain could grow ...
متن کاملThermostable alkaline protease produced by an Aspergillus niger strain
A thermostable alkaline protease was isolated from the Aspergillus niger Z1 strain in a liquid Czapek Dox medium, containing casein (1% w/v) as the sole nitrogen source. Enzyme extract was subjected to electrophoresis in SDS-polyacrylamide gel. Two protein bands were seen on polyacrylamide gel. Active enzyme was visualized in a zymogram and protease activity exhibited a molecular mass of 68 kDa...
متن کاملPurification and Some Properties of a Thermostable Protease, BSP2, Produced from Bacillus stearothermophilus No. 2
A thermophilic bacteria, Uac3l~n.s stl!rtrothe?-rr~oplr iltts No. 2 isolated from sewage sludge compost, was found to produce a thermostable neutral protease (BSP2). The BSP2 was purified from culture broth by fractionation with cold acetone and subsequent column chromatographies. The purified BSP2 gave a single band on SDS PAGE and its molecular weight was estimated to be 35kDa. The purified p...
متن کاملproduction and purification of a protease from an alkalophilic bacillus sp. 2-5 strain isolated from soil
this research has focused on isolation and characterization of a strain of bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at phs ranging from 8 to 11 and temperatures of 20 to 50ºc. also the impact of different carbon andnitrogen sources were investigated. the yield and fold of enzyme purification was 24% and 50 times, respectively. molecular weight o...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1980
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.44.113